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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAPK4 Polyclonal antibody

MAPK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18738-1-AP

Synonyms

ERK 4, ERK3, Erk4, MAP kinase 4, MAP kinase isoform p63, MAPK 4, MAPK4, p63 MAPK, p63MAPK, PRKM4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MAPK4 Polyclonal antibody (18738-1-AP)

    WB analysis of mouse brain using 18738-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18738-1-AP (MAPK4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using MAPK4 Polyclonal antibody (18738-1-AP)

    WB analysis of rat brain using 18738-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 18738-1-AP (MAPK4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using MAPK4 Polyclonal antibody (18738-1-AP)

    IHC staining of human heart using 18738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18738-1-AP (MAPK4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAPK4 Polyclonal antibody (18738-1-AP)

    IHC staining of human heart using 18738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18738-1-AP (MAPK4 Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18738-1-AP targets MAPK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 4
Calculated molecular weight 66 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC117216
Gene symbol MAPK4
Gene ID (NCBI) 5596
RRIDAB_2878559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAPK4 antibody 18738-1-APDownload protocol
IHC protocol for MAPK4 antibody 18738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols