MAPK4 Polyclonal antibody
MAPK4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 26102-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 26102-1-AP is MAPK4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAPK4 fusion protein Ag22899 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase 4 |
| Calculated molecular weight | 587 aa, 66 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC050299 |
| Gene symbol | MAPK4 |
| Gene ID (NCBI) | 5596 |
| RRID | AB_2880378 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAPK4 antibody 26102-1-AP | Download protocol |
| IHC protocol for MAPK4 antibody 26102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Biosci Identification of atypical mitogen-activated protein kinase MAPK4 as a novel regulator in acute lung injury. | ||
J Cell Physiol MiR-127 attenuates adipogenesis by targeting MAPK4 and HOXC6 in porcine adipocytes. | ||
Front Pharmacol An Integrated Proteomics and Bioinformatics Approach Reveals the Anti-inflammatory Mechanism of Carnosic Acid. | ||
Cancer Innov MAPK4 facilitates angiogenesis by inhibiting the ERK pathway in non-small cell lung cancer |
