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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERK5 Polyclonal antibody

ERK5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11164-1-AP

Synonyms

ERK 5, MAPK7, MAPK 7, Extracellular signal-regulated kinase 5, ERK-5

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using ERK5 Polyclonal antibody (11164-1-AP)

    WB analysis of SKOV-3 using 11164-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ERK5 Polyclonal antibody (11164-1-AP)

    WB analysis of mouse heart using 11164-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ERK5 Polyclonal antibody (11164-1-AP)

    WB analysis of HEK-293 using 11164-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ERK5 Polyclonal antibody (11164-1-AP)

    WB analysis of mouse testis using 11164-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11164-1-AP (ERK5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using ERK5 Polyclonal antibody (11164-1-AP)

    IHC staining of human stomach using 11164-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11164-1-AP (ERK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSKOV-3 cells, HEK-293 cells, mouse heart tissue, mouse testis tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11164-1-AP targets ERK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERK5 fusion protein Ag1648 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 7
Calculated molecular weight 89 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC009963
Gene symbol MAPK7
Gene ID (NCBI) 5598
RRIDAB_2877749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ERK5 antibody 11164-1-APDownload protocol
IHC protocol for ERK5 antibody 11164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Authors - Xiulei Mo
View Article