JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 51153-1-AP

Synonyms

MAPK9, JNK2A, JNK2, JNK 55, EC:2.7.11.24


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Tested Applications

Positive WB detected inHeLa cells, A549 cells, COS-7 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51153-1-AP targets JNK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JNK fusion protein Ag1442 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 9
Calculated molecular weight 424 aa, 48 kDa
Observed molecular weight 44-55 kDa
GenBank accession numberBC032539
Gene symbol JNK
Gene ID (NCBI) 5601
RRIDAB_10898168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Protocols

Product Specific Protocols
WB protocol for JNK antibody 51153-1-APDownload protocol
IHC protocol for JNK antibody 51153-1-APDownload protocol
IF protocol for JNK antibody 51153-1-APDownload protocol
IP protocol for JNK antibody 51153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Mater

m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation

Authors - Qing You
humanWB

Cell Rep Med

Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition

Authors - Yu Chang
humanWB

Nat Commun

SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation.

Authors - Peiyuan Zhang
humanWB

Biomed Pharmacother

Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.

Authors - Ya-Sai Sun
ratWB

Neurochem Res

Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK.

Authors - Xuehua Sun
rabbitWB

Microb Pathog

Polysaccharides from Citrus grandis L. Osbeck suppress inflammation and relieve chronic pharyngitis.

Authors - Liyan Chen