Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20993-1-AP targets MAPKAP1 in ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mitogen-activated protein kinase associated protein 1 |
| Calculated molecular weight | 59 kDa |
| GenBank accession number | NM_024117 |
| Gene Symbol | MAPKAP1 |
| Gene ID (NCBI) | 79109 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BPZ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAPKAP1, also named as MIP1 and SIN1, belongs to the SIN1 family. It is a subunit of mTORC2 which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. It inhibits HRAS and KRAS signaling. MAPKAP1 enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. The antibody is specific to MAPKAP1. The antibody can recognize all the isoform of MAPKAP1.