MAPKAPK2 Polyclonal antibody

Name: MAPKAPK2 Polyclonal antibody
Brand: Proteintech
SKU: 13949-1-AP

MAPKAPK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13949-1-AP

Synonyms

MAPKAP-K2, MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 13949-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13949-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 13949-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 13949-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 13949-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13949-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13949-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L); F-actin is stained using CL555-phalloidin (red).
IF Staining of HeLa using 13949-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13949-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, mouse skeletal muscle tissue, mouse colon tissue
Positive IHC detected in	human breast cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IHC	See 2 publications below

Product Information

13949-1-AP targets MAPKAPK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAPKAPK2 fusion protein Ag5060 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase-activated protein kinase 2
Calculated molecular weight	400 aa, 46 kDa
Observed molecular weight	47-50 kDa
GenBank accession number	BC036060
Gene symbol	MAPKAPK2
Gene ID (NCBI)	9261
RRID	AB_2877995
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK2, one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response and is in the nucleus of unstimulated cells and moves rapidly to the cytoplasm after stimulation(PMID:12171911). It is also involved in many other cellular processes including stress responses, nuclear export, gene expression regulation and cell proliferation. Multiple residues of MAPKAPK2 are generally phosphorylated in vivo in response to stress, but only 4 residues(Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in vitro(PMID:22351694). It has 2 isoforms produced by alternative splicing and the range of the molecular weight is 42-60 kDa according to the references(PMID:10666409; 11328854;8995385).

Protocols

Product Specific Protocols
WB protocol for MAPKAPK2 antibody 13949-1-AP	Download protocol
IHC protocol for MAPKAPK2 antibody 13949-1-AP	Download protocol
IF protocol for MAPKAPK2 antibody 13949-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer Res Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2. Authors - Rui Wang View Article
mouse	WB	Food Funct L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice. Authors - Kehong Liu View Article
human	WB	Toxicol Appl Pharmacol p38 MAPK-DRP1 signaling is involved in mitochondrial dysfunction and cell death in mutant A53T α-synuclein model of Parkinson's disease. Authors - Chen Gui View Article
human	WB	Int J Mol Med Aryl hydrocarbon receptor inhibits inflammation in DSS‑induced colitis via the MK2/p‑MK2/TTP pathway. Authors - Qimeng Wang View Article
mouse	WB	Brain Res Bull The p38 mitogen activated protein kinase regulates β-amyloid protein internalization through the α7 nicotinic acetylcholine receptor in mouse brain. Authors - Kai-Ge Ma View Article
mouse	WB	Cell Rep MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal. Authors - Yan Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAPKAPK2 Polyclonal antibody

MAPKAPK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 13949-1-AP

WB analysis of A549 using 13949-1-AP

WB analysis of mouse skeletal muscle using 13949-1-AP

WB analysis of mouse colon using 13949-1-AP

IHC staining of human breast cancer using 13949-1-AP

IHC staining of human breast cancer using 13949-1-AP

IHC staining of human kidney using 13949-1-AP

IHC staining of human kidney using 13949-1-AP

IF Staining of HeLa using 13949-1-AP

IF Staining of HeLa using 13949-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2.

L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.

p38 MAPK-DRP1 signaling is involved in mitochondrial dysfunction and cell death in mutant A53T α-synuclein model of Parkinson's disease.

Aryl hydrocarbon receptor inhibits inflammation in DSS‑induced colitis via the MK2/p‑MK2/TTP pathway.

The p38 mitogen activated protein kinase regulates β-amyloid protein internalization through the α7 nicotinic acetylcholine receptor in mouse brain.

MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal.