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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MAPKAPK2 Recombinant antibody

MAPKAPK2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230197D6

Cat no : CL488-82929-2

Synonyms

MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2, EC:2.7.11.1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated MAPKAPK2 Recombinant (CL488-82929-2)

    FC experiment of HeLa using CL488-82929-2

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Mapkapk2 Recombinant Antibody (CL488-82929-2, Clone:230197D6)(red), or 0.25 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-82929-2 is MAPKAPK2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAPKAPK2 fusion protein Ag34196 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase-activated protein kinase 2
Calculated molecular weight 400 aa, 46 kDa
Observed molecular weight42-46 kDa
GenBank accession numberBC036060
Gene symbol MAPKAPK2
Gene ID (NCBI) 9261
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 MAPKAPK2 antibody CL488-82929-2Download protocol
Standard Protocols
Click here to view our Standard Protocols