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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAPKAPK3 Polyclonal antibody

MAPKAPK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15424-1-AP

Synonyms

MAPKAP kinase 3, MAPK-activated protein kinase 3, MAP kinase-activated protein kinase 3, EC:2.7.11.1, Chromosome 3p kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MAPKAPK3 Polyclonal antibody (15424-1-AP)

    WB analysis of mouse skeletal muscle using 15424-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15424-1-AP (MAPKAPK3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MAPKAPK3 Polyclonal antibody (15424-1-AP)

    IHC staining of human placenta using 15424-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MAPKAPK3 Polyclonal antibody (15424-1-AP)

    IHC staining of human placenta using 15424-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAPKAPK3 Polyclonal antibody (15424-1-AP)

    IF Staining of HeLa using 15424-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15424-1-AP targets MAPKAPK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPKAPK3 fusion protein Ag7875 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase-activated protein kinase 3
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001662
Gene symbol MAPKAPK3
Gene ID (NCBI) 7867
RRIDAB_2878136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
IHC protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
IF protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Bioengineered

Inhibition of translocator protein 18 kDa suppressed the progression of glioma via the ELAV-like RNA-binding protein 1/MAPK-activated protein kinase 3 axis.

Authors - Jingya Wang
View Article
ratWB,IF

Front Neurosci

Identification of key genes and pathways revealing the central regulatory mechanism of brain-derived glucagon-like peptide-1 on obesity using bioinformatics analysis

Authors - Yuwei Shao
View Article