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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAPKSP1 Polyclonal antibody

MAPKSP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11937-1-AP

Synonyms

LAMTOR3, MAP2K1IP1, MAPBP, MAPK scaffold protein 1, MAPKSP1, MEK binding partner 1, MP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human liver tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    WB analysis of human liver using 11937-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    WB analysis of mouse liver using 11937-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using MAPKSP1 Polyclonal antibody (11937-1-AP)

    WB analysis of L02 using 11937-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    WB analysis of mouse brain using 11937-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human prostate cancer using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human heart using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human heart using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human liver using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human liver using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human prostate cancer using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human prostate cancer using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman liver tissue, L02 cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman prostate cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11937-1-AP targets MAPKSP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPKSP1 fusion protein Ag2534 相同性解析による交差性が予測される生物種
Full Name MAPK scaffold protein 1
Calculated molecular weight 124 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC026245
Gene symbol MAPKSP1
Gene ID (NCBI) 8649
RRIDAB_2141610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPKSP1, also named as mitogen activated protein kinase kinase 1 interacting protein 1, Map2k1ip1, MEK partner 1, Lamtor3 and MP1. It was first identified as a scaffold protein enhancing the efficiency of the MAPK pathway by facilitating the interaction between MEK1 and ERK1 upon serum stimulation (PMID: 9733512). Later, it has been demonstrated that MAPKSP1 enhances activation of both ERK1 and ERK2 as a response to EGF (PMID: 12479806) and fibronectin (PMID: 15923628). MAPKSP1 is involved in regulating endosomal signaling (PMID: 17178906), and a role in cancer, via MEK and ERK hyperactivation, has been demostrated in pancreatic tumorigenesis (PMID: 24209743). MAPKSP1 has two isoforms with the molecular weight of 13-14 kDa.

Protocols

Product Specific Protocols
WB protocol for MAPKSP1 antibody 11937-1-APDownload protocol
IHC protocol for MAPKSP1 antibody 11937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Pharmacol

Brucea javanica Oil Emulsion Promotes Autophagy in Ovarian Cancer Cells Through the miR-8485/LAMTOR3/mTOR/ATG13 Signaling Axis

Authors - Yihan Wang
View Article