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EB1 Polyclonal antibody

Name: EB1 Polyclonal antibody
Brand: Proteintech
SKU: 17717-1-AP

EB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17717-1-AP

Synonyms

MAPRE1, Microtubule-associated protein RP/EB family member 1, End-binding protein 1, End binding protein 1, APC-binding protein EB1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa using 17717-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17717-1-AP
WB result of EB1 antibody (17717-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EB1 transfected HeLa cells.
WB analysis of Jurkat using 17717-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 17717-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17717-1-AP
IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with HeLa cells lysate 1200ug.
IP experiment of mouse thymus using 17717-1-AP
IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with mouse thymus tissue lysate 2800ug.
IP experiment of Caco-2 using 17717-1-AP
IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with Caco-2 cells lysate 1920 ug.
IHC staining of human kidney using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17717-1-AP (EB1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17717-1-AP (EB1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 17717-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 17717-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EB1 antibody (17717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 17717-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EB1 (17717-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 17717-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, A431 cells
Positive IP detected in	HeLa cells, Caco-2 cells, mouse thymus tissue
Positive IHC detected in	human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human normal colon, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 4 publications below

Product Information

17717-1-AP targets EB1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EB1 fusion protein Ag12092 相同性解析による交差性が予測される生物種
Full Name	microtubule-associated protein, RP/EB family, member 1
Calculated molecular weight	268 aa, 30 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC106068
Gene symbol	EB1
Gene ID (NCBI)	22919
RRID	AB_2141624
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EB1 antibody 17717-1-AP	Download protocol
IHC protocol for EB1 antibody 17717-1-AP	Download protocol
IF protocol for EB1 antibody 17717-1-AP	Download protocol
IP protocol for EB1 antibody 17717-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Authors - Hai-Qing Tu View Article
human	IF	Elife α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization. Authors - Dandan Ma View Article
mouse	IF	Biol Open MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1 Authors - Peihong Su View Article
mouse	IF	Biol Open MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1. Authors - Peihong Su View Article
human	IF	Molecules Pyrrolyldihydropyrazino[1,2-a]indoletrione Analogue Microtubule Inhibitor Induces Cell-Cycle Arrest and Apoptosis in Colorectal Cancer Cells Authors - Dong-Lin Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EB1 Polyclonal antibody

EB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 17717-1-AP

WB analysis of HeLa using 17717-1-AP

WB analysis of Jurkat using 17717-1-AP

WB analysis of A431 using 17717-1-AP

IP experiment of HeLa using 17717-1-AP

IP experiment of mouse thymus using 17717-1-AP

IP experiment of Caco-2 using 17717-1-AP

IHC staining of human kidney using 17717-1-AP

IHC staining of human kidney using 17717-1-AP

IHC staining of human colon using 17717-1-AP

IHC staining of human colon using 17717-1-AP

IHC staining of human colon cancer using 17717-1-AP

IHC staining of human colon cancer using 17717-1-AP

IHC staining of human placenta using 17717-1-AP

IHC staining of human placenta using 17717-1-AP

IHC staining of human testis using 17717-1-AP

IHC staining of human testis using 17717-1-AP

IHC staining of human skin using 17717-1-AP

IHC staining of human skin using 17717-1-AP

IHC staining of human spleen using 17717-1-AP

IHC staining of human spleen using 17717-1-AP

IHC staining of human lung using 17717-1-AP

IHC staining of human lung using 17717-1-AP

IF Staining of HeLa using 17717-1-AP

FC experiment of HeLa using 17717-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.

MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1

MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1.

Pyrrolyldihydropyrazino[1,2-a]indoletrione Analogue Microtubule Inhibitor Induces Cell-Cycle Arrest and Apoptosis in Colorectal Cancer Cells