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  • Featured Product
  • KD/KO Validated

MAPRE2 Polyclonal antibody

MAPRE2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10364-1-AP

Synonyms

RP1, Microtubule-associated protein RP/EB family member 2, End-binding protein 2, EB2, APC-binding protein EB2

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using MAPRE2 Polyclonal antibody (10364-1-AP)

    WB analysis of K-562 using 10364-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10364-1-AP (MAPRE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MAPRE2 Polyclonal antibody (10364-1-AP)

    WB analysis of Jurkat using 10364-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10364-1-AP (MAPRE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using MAPRE2 Polyclonal antibody (10364-1-AP)

    IHC staining of human stomach using 10364-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10364-1-AP (MAPRE2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cells using MAPRE2 Polyclonal antibody (10364-1-AP)

    IF Staining of mouse cells using 10364-1-AP

    Mouse keratinocyte showing MAPRE2 (#10364-1-AP, 1:100 dilution, with Alexa 594 labelled 2nd Ab) in red and alpha-tubulin (#66031-1-lg, 1:100 dilution, with Alexa 488 labelled 2nd Ab ) in green.

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Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10364-1-AP targets MAPRE2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPRE2 fusion protein Ag0416 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein, RP/EB family, member 2
Calculated molecular weight 37 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC007318
Gene symbol MAPRE2
Gene ID (NCBI) 10982
RRIDAB_2141649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RP1 protein shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family and binds specifically to the C-terminal region of the APC protein. RP1 also binds directly to tubulin, both in vitro and in vivo. The function of RP1 is currently unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. It may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Protocols

Product Specific Protocols
WB protocol for MAPRE2 antibody 10364-1-APDownload protocol
IHC protocol for MAPRE2 antibody 10364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
View Article
mouseWB

Exp Cell Res

MAPRE2 regulates the first meiotic progression in mouse oocytes.

Authors - Yuan-Yuan Li
  • KD Validated
View Article