EB3 Polyclonal antibody

Name: EB3 Polyclonal antibody
Brand: Proteintech
SKU: 23974-1-AP

EB3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 23974-1-AP

Synonyms

EB3, End binding protein 3, MAPRE3

Validation Data Gallery

Filter:

AllWBIHCIF-P

WB analysis of mouse brain using 23974-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 23974-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 23974-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 23974-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 23974-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23974-1-AP (EB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 23974-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EB3 antibody (23974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 23974-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IHC detected in	human skeletal muscle tissue, human brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 1 publications below

Product Information

The immunogen of 23974-1-AP is EB3 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EB3 fusion protein Ag21148 相同性解析による交差性が予測される生物種
Full Name	microtubule-associated protein, RP/EB family, member 3
Calculated molecular weight	32 kDa
Observed molecular weight	30-36 kDa
GenBank accession number	BC011557
Gene symbol	EB3
Gene ID (NCBI)	22924
RRID	AB_2879385
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EB3 antibody 23974-1-AP	Download protocol
IHC protocol for EB3 antibody 23974-1-AP	Download protocol
IF protocol for EB3 antibody 23974-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Authors - Hai-Qing Tu View Article
mouse	WB	Cell Tissue Res Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress Authors - Valentina Grande View Article
rat	IF	Neural Regen Res The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons Authors - Shi-Yan Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EB3 Polyclonal antibody

EB3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis of mouse brain using 23974-1-AP

WB analysis of mouse brain using 23974-1-AP

WB analysis of mouse brain using 23974-1-AP

WB analysis of rat brain using 23974-1-AP

IHC staining of human skeletal muscle using 23974-1-AP

IHC staining of human skeletal muscle using 23974-1-AP

IHC staining of human brain using 23974-1-AP

IHC staining of human brain using 23974-1-AP

IHC staining of mouse brain using 23974-1-AP

IF Staining of mouse testis using 23974-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons