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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MARCH5 Polyclonal antibody (12213-1-AP)

    WB analysis of HeLa using 12213-1-AP

    WB result of MARCH5 antibody (12213-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MARCH5 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using MARCH5 Polyclonal antibody (12213-1-AP)

    WB analysis using 12213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12213-1-AP (MARCH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MARCH5 Polyclonal antibody (12213-1-AP)

    IHC staining of human skeletal muscle using 12213-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MARCH5 Polyclonal antibody (12213-1-AP)

    IHC staining of human skeletal muscle using 12213-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12213-1-AP (MARCH5 Antibody) at dilution of 1:50 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

MARCH5 Polyclonal antibody

MARCH5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12213-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MARCH V, MARCH5, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, RNF153

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, U-937 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12213-1-AP targets MARCH5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARCH5 fusion protein Ag2806 相同性解析による交差性が予測される生物種
Full Name membrane-associated ring finger (C3HC4) 5
Calculated molecular weight 278 aa, 31 kDa
Observed molecular weight 31 kDa, 65-70 kDa
GenBank accession numberBC015480
Gene Symbol MARCH5
Gene ID (NCBI) 54708
RRIDAB_10638602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MARCH5(E3 ubiquitin-protein ligase MARCH5) is a 31 kDa protein also named as RNF153. This protein plays a protective role against mitochondrial dysfunction caused by the mitochondrial accumulation of mSOD1 via the ubiquitin-proteasome pathway (PMID:19741096). And it may regulate MAP1B LC1 function in mitochondria by controlling mitochondrial aggregation via mitochondrial LC1 levels(PMID:22308378). MEARCH5 can exsit as a homodimer with MW 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for MARCH5 antibody 12213-1-APDownload protocol
IHC protocol for MARCH5 antibody 12213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Med

FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.

Authors - Lei Miao
View Article