Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51087-1-AP targets MARCKS in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | myristoylated alanine-rich protein kinase C substrate |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 32 kDa, 80 kDa |
| GenBank accession number | BC089040 |
| Gene Symbol | MARCKS |
| Gene ID (NCBI) | 4082 |
| RRID | AB_2881243 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29966 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
MARCKS(Myristoylated alanine-rich C-kinase substrate, 32 kDa ) is also named as MACS, PRKCSL and belongs to the MARCKS family. It is also named as protein kinase C substrate(80 kDa protein, light chain). MARCKS is a filamentous actincrosslinking protein that appears to function as an integrator of protein kinase C and calcium (Ca2+)/calmodulin signals in the regulation of actin-membrane interactions. This protein can be Phosphorylated by PKC displaces MARCKS from the membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MARCKS antibody 51087-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |