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KD/KO Validated

MARK1 Polyclonal antibody

Name: MARK1 Polyclonal antibody
Brand: Proteintech
SKU: 21552-1-AP
Rating: 5.00 (1 reviews)

MARK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 21552-1-AP

Synonyms

Par1c, PAR1 homolog c, MARK, MAP/microtubule affinity-regulating kinase 1, MAP/microtubule affinity regulating kinase 1

Validation Data Gallery

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AllWBIPIHC

WB analysis using 21552-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21552-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21552-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21552-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 21552-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 21552-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 21552-1-AP
IP result of anti-MARK1 (IP:21552-1-AP, 4ug; Detection:21552-1-AP 1:1000) with mouse brain tissue lysate 3440ug.
IHC staining of rat testis using 21552-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21552-1-AP (MARK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 21552-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21552-1-AP (MARK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 21552-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, SH-SY5Y cells, mouse kidney tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 9 publications below
IP	See 2 publications below

Product Information

21552-1-AP targets MARK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MARK1 fusion protein Ag16225 相同性解析による交差性が予測される生物種
Full Name	MAP/microtubule affinity-regulating kinase 1
Calculated molecular weight	795 aa, 89 kDa
Observed molecular weight	85-89 kDa, 72 kDa
GenBank accession number	BC113869
Gene symbol	MARK1
Gene ID (NCBI)	4139
RRID	AB_10732726
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MARK1, also named as MAP-microtubule affinity regulating kinase 1, which form a subfamily of the calcium/calmodulin-dependent protein kinase (CAMP) group of kinases(PMID: 22670221). It is involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1 also act as a regulator of the neuronal migration and Wnt signaling pathway. It has 3 isoforms which molecular weight is 89,84,72 kDa. This antibody may detect all of the isoforms.

Protocols

Product Specific Protocols
WB protocol for MARK1 antibody 21552-1-AP	Download protocol
IHC protocol for MARK1 antibody 21552-1-AP	Download protocol
IP protocol for MARK1 antibody 21552-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential. Authors - Jonathan M Goodwin KD Validated View Article
human	IP	Nat Commun TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth. Authors - Tsz-Yin Chan KD Validated View Article
mouse	WB	Cell Rep DIXDC1 Phosphorylation and Control of Dendritic Morphology Are Impaired by Rare Genetic Variants. Authors - Vickie Kwan View Article
human	WB	Cell Commun Signal Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. Authors - Abiola Abdulrahman Ayanlaja View Article
rat	WB,IP	J Biol Chem Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity. Authors - Qian Wu View Article
rat	WB	PLoS One MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism. Authors - Laura P Bernard View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Manish (Verified Customer) (06-09-2020)

The antibody works really well. We dilute it 10 fold and still get strong bands on western blots.

Applications: Western Blot
Primary Antibody Dilution: 1:1000

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MARK1 Polyclonal antibody

MARK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 21552-1-AP

WB analysis of HeLa using 21552-1-AP

WB analysis of mouse brain using 21552-1-AP

WB analysis of HeLa using 21552-1-AP

WB analysis of SH-SY5Y using 21552-1-AP

WB analysis of mouse kidney using 21552-1-AP

IP experiment of mouse brain using 21552-1-AP

IHC staining of rat testis using 21552-1-AP

IHC staining of rat testis using 21552-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.

TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.

DIXDC1 Phosphorylation and Control of Dendritic Morphology Are Impaired by Rare Genetic Variants.

Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.

MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism.

Reviews