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  • KD/KO Validated

MARS Polyclonal antibody

MARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14829-1-AP

Synonyms

MARS1, EC:6.1.1.10, MTRNS, METRS, Methionyl-tRNA synthetase


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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14829-1-AP targets MARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARS fusion protein Ag6619 相同性解析による交差性が予測される生物種
Full Name methionyl-tRNA synthetase
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC002384
Gene symbol MARS
Gene ID (NCBI) 4141
RRIDAB_2140917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MARS antibody 14829-1-APDownload protocol
IHC protocol for MARS antibody 14829-1-APDownload protocol
IF protocol for MARS antibody 14829-1-APDownload protocol
IP protocol for MARS antibody 14829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging Cell

N-homocysteinylation of DJ-1 promotes neurodegeneration in Parkinson's disease

Authors - Tao Guo
humanWB

Proteomics

Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.

Authors - Jing Zhang
humanWB,IHC

J Clin Pathol

High methionyl-tRNA synthetase expression predicts poor prognosis in patients with breast cancer.

Authors - Qin Jin
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex

Authors - Krishnendu Khan
  • KD Validated
mouseWB

Aging Cell

N-homocysteinylation of α-synuclein promotes its aggregation and neurotoxicity

Authors - Lingyan Zhou
  • KD Validated