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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MARS Monoclonal antibody, PBS Only

MARS Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E3B4

Cat no : 67739-1-PBS

Synonyms

FLJ35667, MARS, Methionine tRNA ligase, methionyl tRNA synthetase, METRS, MTRNS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MARS Monoclonal antibody (67739-1-Ig)

    WB analysis using 67739-1-Ig (same clone as 67739-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67739-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using MARS Monoclonal antibody (67739-1-Ig)

    WB analysis of A431 using 67739-1-Ig (same clone as 67739-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67739-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MARS Monoclonal antibody (67739-1-Ig)

    IF Staining of HepG2 using 67739-1-Ig (same clone as 67739-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (67739-1-Ig, Clone: 3E3B4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67739-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67739-1-PBS targets MARS in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MARS fusion protein Ag6961 相同性解析による交差性が予測される生物種
Full Name methionyl-tRNA synthetase
Calculated molecular weight 101 kDa
Observed molecular weight 97-101 kDa
GenBank accession numberBC002384
Gene symbol MARS
Gene ID (NCBI) 4141
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.