Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human small intestine tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
13515-1-AP targets MARVELD2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MARVELD2 fusion protein Ag4446 相同性解析による交差性が予測される生物種 |
| Full Name | MARVEL domain containing 2 |
| Calculated molecular weight | 558 aa, 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC033689 |
| Gene Symbol | MARVELD2 |
| Gene ID (NCBI) | 153562 |
| RRID | AB_2281702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N4S9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MARVELD2, also named as Tricellulin or TRIC, is a 558 amino acid protein, which contains one MARVEL domain. MARVELD2 localizes the cell membrane and plays a role in the formation of the epithelial barriers. MARVELD2, is a first integral membrane protein that is concentrated at the vertically oriented tight junction strands of tricellular contacts. MARVELD2 may be a component to maintain the integrity for peripheral nervous system myelin function and morphology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MARVELD2 antibody 13515-1-AP | Download protocol |
| IHC protocol for MARVELD2 antibody 13515-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Tricellulin deficiency affects tight junction architecture and cochlear hair cells. | ||
Microb Pathog Alterations in the expression level of visfatin in the lungs of piglets infected with PRRSV and its effect on PRRSV replication. | ||
PLoS One Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon | ||
Tissue Cell Papilla cells may guard the entrance to ampullary organs of Polyodon electroreceptors | ||
Cell Rep Alpha-tocopherylquinone differentially modulates claudins to enhance intestinal epithelial tight junction barrier via AhR and Nrf2 pathways |