MARVELD3 Polyclonal antibody
MARVELD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 25667-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
The immunogen of 25667-1-AP is MARVELD3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MARVELD3 fusion protein Ag22492 相同性解析による交差性が予測される生物種 |
| Full Name | MARVEL domain containing 3 |
| Calculated molecular weight | 401 aa, 45 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC013376 |
| Gene symbol | MARVELD3 |
| Gene ID (NCBI) | 91862 |
| RRID | AB_2880184 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MARVELD3 (MARVEL domain-containing protein 3), a four-span transmembrane protein of about 40 kDa, is a tight junction-associated transmembrane protein of the occludin family (PMID: 20028514). MARVELD3 functions as a determinant of epithelial paracellular permeability properties. It has been reported that MARVELD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behavior and survival (PMID: 24567356).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| IHC protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| IF protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Molecular weight-dependent hyaluronic acid permeability and tight junction modulation in human buccal TR146 cell monolayers | ||
FEBS Open Bio Hesperidin enhances intestinal barrier function in Caco-2 cell monolayers via AMPK-mediated tight junction-related proteins |
