MAT1A/MAT2A Polyclonal antibody

MAT1A/MAT2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, arabidopsis

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12395-1-AP

Synonyms

AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1


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Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, HCT 116 cells, arabidopsis whole plant tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12395-1-AP targets MAT1A/MAT2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, arabidopsis samples.

Tested Reactivity human, mouse, rat, arabidopsis
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAT1A/MAT2A fusion protein Ag3076 相同性解析による交差性が予測される生物種
Full Name methionine adenosyltransferase I, alpha
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight 44-55 kDa
GenBank accession numberBC018359
Gene symbol MAT1A
Gene ID (NCBI) 4143
RRIDAB_2266186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and can recognize both of MAT1A and MAT2A. Western blot analysis using this antibody detected a single band around 44-50 kDa in liver.

Protocols

Product Specific Protocols
WB protocol for MAT1A/MAT2A antibody 12395-1-APDownload protocol
IHC protocol for MAT1A/MAT2A antibody 12395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Ann N Y Acad Sci

PMID: 29722030

Authors - Holly M Brown-Borg
humanWB

Am J Pathol

Forced expression of methionine adenosyltransferase 1A in human hepatoma cells suppresses in vivo tumorigenicity in mice.

Authors - Li Jiaping J
ratWB

Toxicol Sci

Metabolomic Analysis Reveals Metabolic Changes Caused By Bisphenol A in Rats.

Authors - Minjian Chen
mouseWB

Proteomics

Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.

Authors - Thomas Anja A
mouseWB

Mech Ageing Dev

Sulfur-based redox alterations in long-lived Snell dwarf mice.

Authors - Vitvitsky Victor V
humanIHC

J Surg Oncol

Histological expression of methionine adenosyl transferase (MAT) 2A as a post-surgical prognostic surrogate in patients with hepatocellular carcinoma.

Authors - Jihyun An

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (03-26-2021)

Great antibody

  • Applications: Western Blot
  • Cell Tissue Type: Mouse liver, mouse brain / HepG2
MAT1A/MAT2A Antibody Western Blot validation ( dilution) in Mouse liver, mouse brain / HepG2 (Cat no:12395-1-AP)