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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAT1A Monoclonal antibody (67408-1-Ig)

    WB analysis using 67408-1-Ig (same clone as 67408-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67408-1-Ig (MAT1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67408-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAT1A Monoclonal antibody (67408-1-Ig)

    IF Staining of HeLa using 67408-1-Ig (same clone as 67408-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAT1A antibody (67408-1-Ig, Clone: 2F1C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67408-1-PBS in a different storage buffer formulation.

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MAT1A Monoclonal antibody, PBS Only

MAT1A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67408-1-PBS
Clone No.2F1C1

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67408-1-PBS targets MAT1A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MAT1A fusion protein Ag3076 相同性解析による交差性が予測される生物種
Full Name methionine adenosyltransferase I, alpha
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC018359
Gene Symbol MAT1A
Gene ID (NCBI) 4143
RRIDAB_2882649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00266
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A.