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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MATN1 Polyclonal antibody (28566-1-AP)

    WB analysis using 28566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28566-1-AP (MATN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MATN1 Polyclonal antibody (28566-1-AP)

    IF Staining of HeLa using 28566-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MATN1 antibody (28566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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MATN1 Polyclonal antibody

MATN1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28566-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

pig, human

Applications

WB, IF/ICC, ELISA

Synonyms

Cartilage matrix protein, CMP, CRTM, MATN1, Matrilin 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig cartilage tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28566-1-AP targets MATN1 in WB, IF/ICC, ELISA applications and shows reactivity with pig, human samples.

Tested Reactivity pig, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MATN1 fusion protein Ag29386 相同性解析による交差性が予測される生物種
Full Name matrilin 1, cartilage matrix protein
Calculated molecular weight 54 kDa
Observed molecular weight54 kDa
GenBank accession numberNM_002379
Gene Symbol MATN1
Gene ID (NCBI) 4146
RRIDAB_3669662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21941
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MATN1 (matrilin 1), also known as CMP. It is expected to be located in extracellular space, the protein is mainly expressed in cartilage tissue and retina tissue. Also, the protein is a member of von Willebrand factor A domain containing protein family. This family of proteins are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. Mutations of this gene have been associated with variety of inherited chondrodysplasias. It is reproted that COMP and matrilin-1 elute together in the gel filtration of cartilage extracts and can be co-immunoprecipitated with the presence of calcium (PMID: 15075323). The calculated molecular weight of MATN1 is 54 kDa.

Protocols

Product Specific Protocols
WB protocol for MATN1 antibody 28566-1-APDownload protocol
IF protocol for MATN1 antibody 28566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols