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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse embryo tissue using MATN1 Recombinant antibody (84313-1-RR)

    WB analysis of mouse embryo using 84313-1-RR

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 84313-1-RR (MATN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84313-1-RR

    Affinity and Kinetic Characterization of 84313-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84313-1-RR against Human MATN1 were performed. The affinity constant is 1.14 nM.

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MATN1 Recombinant antibody

MATN1 Recombinant Antibody for WB, ELISA
Cat No. 84313-1-RR
Clone No.241643D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Matrilin 1, CRTM, CMP, Cartilage matrix protein, 241643D1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse embryo tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84313-1-RR targets MATN1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MATN1 fusion protein Ag29386 相同性解析による交差性が予測される生物種
Full Name matrilin 1, cartilage matrix protein
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_002379
Gene Symbol MATN1
Gene ID (NCBI) 4146
RRIDAB_3671854
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21941
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MATN1 (matrilin 1), also known as CMP. It is expected to be located in extracellular space, the protein is mainly expressed in cartilage tissue and retina tissue. Also, the protein is a member of von Willebrand factor A domain containing protein family. This family of proteins are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. Mutations of this gene have been associated with variety of inherited chondrodysplasias. It is reproted that COMP and matrilin-1 elute together in the gel filtration of cartilage extracts and can be co-immunoprecipitated with the presence of calcium (PMID: 15075323). The calculated molecular weight of MATN1 is 54 kDa.

Protocols

Product Specific Protocols
WB protocol for MATN1 antibody 84313-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols