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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MATN3 Polyclonal antibody

MATN3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25500-1-AP

Synonyms

Matrilin-3, matrilin 3, HOA, EDM5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MATN3 Polyclonal antibody (25500-1-AP)

    WB analysis using 25500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25500-1-AP (MATN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MATN3 Polyclonal antibody (25500-1-AP)

    IHC staining of human stomach cancer using 25500-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25500-1-AP (MATN3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman milk
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25500-1-AP targets MATN3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MATN3 fusion protein Ag21932 相同性解析による交差性が予測される生物種
Full Name matrilin 3
Calculated molecular weight 486 aa, 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC139907
Gene symbol MATN3
Gene ID (NCBI) 4148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. It is linked to the development of many types of cartilage, and part of the Matrilin family, which includes Matrilin-1, Matrilin-2, Matrilin-3, and Matrilin-4, a family of filamentous-forming adapter oligomeric extracellular proteins that are linked to the formation of cartilage and bone, as well as maintaining homeostasis after development. It is considered an extracellular matrix protein that functions as an adapter protein where the Matrilin-3 subunit can form both homo-tetramers and hetero-oligomers with subunits from Matrilin-1 which is the cartilage matrix protein. This restricted tissue has been strongly expressed in growing skeletal tissue as well as cartilage and bone.( PMID:26499313, 27104523, 11950247)

Protocols

Product Specific Protocols
WB protocol for MATN3 antibody 25500-1-APDownload protocol
IHC protocol for MATN3 antibody 25500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols