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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MATR3 Monoclonal antibody (68280-1-Ig)

    WB analysis using 68280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using MATR3 Monoclonal antibody (68280-1-Ig)

    WB analysis using 68280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68280-1-Ig (MATR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MATR3 Monoclonal antibody (68280-1-Ig)

    IF Staining of HEK-293 using 68280-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (68280-1-Ig, Clone: 3B12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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MATR3 Monoclonal antibody

MATR3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68280-1-Ig
Clone No.3B12C7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Matrin-3, matrin 3, KIAA0723

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, rat brain tissue, A431 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, mouse brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68280-1-Ig targets MATR3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MATR3 fusion protein Ag31502 相同性解析による交差性が予測される生物種
Full Name matrin 3
Calculated molecular weight 847 aa, 95 kDa
Observed molecular weight120 kDa
GenBank accession numberBC015031
Gene Symbol MATR3
Gene ID (NCBI) 9782
RRIDAB_2935361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43243
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.

Protocols

Product Specific Protocols
WB protocol for MATR3 antibody 68280-1-IgDownload protocol
IF protocol for MATR3 antibody 68280-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols