Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| ChIP | See 4 publications below |
Product Information
10426-1-AP targets MAX in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAX fusion protein Ag0680 相同性解析による交差性が予測される生物種 |
| Full Name | MYC associated factor X |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC003525 |
| Gene Symbol | MAX |
| Gene ID (NCBI) | 4149 |
| RRID | AB_2141660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61244 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAX antibody 10426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.
| ||
Sci Adv A MYC inhibitor selectively alters the MYC and MAX cistromes and modulates the epigenomic landscape to regulate target gene expression. | ||
Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. | ||
Elife Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness. | ||
Cell Commun Signal Novel dual-targeting c-Myc inhibitor D347-2761 represses myeloma growth via blocking c-Myc/Max heterodimerization and disturbing its stability. | ||
J Biol Chem Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1. |