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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAZ Polyclonal antibody

MAZ Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21068-1-AP

Synonyms

Purine-binding transcription factor, Pur-1, PUR1, Pur 1, Myc-associated zinc finger protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MAZ Polyclonal antibody (21068-1-AP)

    WB analysis of HeLa using 21068-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MAZ Polyclonal antibody (21068-1-AP)

    WB analysis of HeLa using 21068-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using MAZ Polyclonal antibody (21068-1-AP)

    WB analysis of mouse lung using 21068-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MAZ Polyclonal antibody (21068-1-AP)

    IF Staining of A431 using 21068-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAZ antibody (21068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21068-1-AP targets MAZ in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAZ fusion protein Ag15200 相同性解析による交差性が予測される生物種
Full Name MYC-associated zinc finger protein (purine-binding transcription factor)
Calculated molecular weight 477 aa, 49 kDa
Observed molecular weight 49-55 kDa
GenBank accession numberBC041629
Gene symbol MAZ
Gene ID (NCBI) 4150
RRIDAB_2878805
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAZ antibody 21068-1-APDownload protocol
IF protocol for MAZ antibody 21068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

TUG1/MAZ/FTH1 Axis Attenuates the Antiglioma Effect of Dihydroartemisinin by Inhibiting Ferroptosis

Authors - Hailong Gong
View Article
mouseIF

Cell Rep

Multi-omics profiling visualizes dynamics of cardiac development and functions

Authors - Yayun Gu
View Article