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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAZ Recombinant antibody

MAZ Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5

Cat no : 82852-3-RR

Synonyms

Pur-1, PUR1, Pur 1, Myc-associated zinc finger protein, MAZI

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAZ Recombinant antibody (82852-3-RR)

    IHC staining of human prostate cancer using 82852-3-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82852-3-RR (MAZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAZ Recombinant antibody (82852-3-RR)

    IHC staining of human prostate cancer using 82852-3-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82852-3-RR (MAZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MAZ Recombinant antibody (82852-3-RR)

    IF Staining of U2OS using 82852-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAZ antibody (82852-3-RR, Clone: 1D5 ) at dilution of 1:950 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAZ Recombinant antibody (82852-3-RR)

    IF Staining of HeLa using 82852-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAZ antibody (82852-3-RR, Clone: 1D5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82852-3-RR

    Affinity and Kinetic Characterization of 82852-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82852-3-RR against Human MAZ were performed. The affinity constant is 0.776 nM.

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Tested Applications

Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82852-3-RR targets MAZ in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAZ fusion protein Ag15200 相同性解析による交差性が予測される生物種
Full Name MYC-associated zinc finger protein (purine-binding transcription factor)
Calculated molecular weight 477 aa, 49 kDa
Observed molecular weight 49-51 kDa
GenBank accession numberBC041629
Gene symbol MAZ
Gene ID (NCBI) 4150
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myc-associated zinc-finger protein (MAZ) is a transcription factor with dual roles in transcription initiation and termination. MAZ can activate the expression of a variety of genes such as the c-Myc, insulin, VEGF, and RAS gene family and the serotonin 1A receptor. MAZ plays a critical role in the progression of various cancers including breast, prostate, liver, and so on. Deregulation of MAZ expression is associated with the progression of pancreatic ductal adenocarcinoma (PDAC)(PMID: 29414775).

Protocols

Product Specific Protocols
IHC protocol for MAZ antibody 82852-3-RRDownload protocol
IF protocol for MAZ antibody 82852-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols