Validation Data Gallery

Filter: AllWBIHC

WB analysis of Jurkat using 55200-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55200-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55200-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55200-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 55200-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 55200-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 55200-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 55200-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 55200-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 55200-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

MBD2 Polyclonal antibody

MBD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55200-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Demethylase, DMTase, MBD2, NY CO 41

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, human brain tissue, A431 cells, HEK-293 cells
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IF	See 1 publications below

Product Information

55200-1-AP targets MBD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	methyl-CpG binding domain protein 2
Calculated molecular weight	43 kDa
Observed molecular weight	43-45 kDa
GenBank accession number	NM_003927
Gene Symbol	MBD2
Gene ID (NCBI)	8932
RRID	AB_10852963
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBB5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.

Protocols

Product Specific Protocols
WB protocol for MBD2 antibody 55200-1-AP	Download protocol
IHC protocol for MBD2 antibody 55200-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. Authors - Xiao-Hui Wang KD Validated View Article
mouse	WB	Exp Cell Res Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway. Authors - Ling Cheng KO Validated View Article
human	WB	Cell Rep TRPS1 drives heterochromatic origin refiring and cancer genome evolution. Authors - Jianguo Yang View Article
human	WB	Genet Test Mol Biomarkers The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas. Authors - Mei-Rong Liang View Article
human	WB,IF	Gene Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database Authors - Jianjun Wu View Article
human	WB	Nucleic Acids Res CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development Authors - Xue Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 55200-1-AP

WB analysis of A431 using 55200-1-AP

WB analysis of A431 using 55200-1-AP

WB analysis of A431 using 55200-1-AP

WB analysis of HEK-293 using 55200-1-AP

WB analysis of human brain using 55200-1-AP

WB analysis of human brain using 55200-1-AP

WB analysis of human brain using 55200-1-AP

IHC staining of human cervical cancer using 55200-1-AP

IHC staining of human cervical cancer using 55200-1-AP

MBD2 Polyclonal antibody

MBD2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.

Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database

CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development