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  • KD/KO Validated

MBD5 Polyclonal antibody

MBD5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15961-1-AP

Synonyms

KIAA1461, MBD5, MRD1


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Tested Applications

Positive WB detected inmouse heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15961-1-AP targets MBD5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBD5 fusion protein Ag8728 相同性解析による交差性が予測される生物種
Full Name methyl-CpG binding domain protein 5
Calculated molecular weight 292 aa, 31 kDa, 160 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC014534
Gene symbol MBD5
Gene ID (NCBI) 55777
RRIDAB_2281588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In vertebrates, cytosine methylation in DNA is one of the major epigenetic modifications, which regulates many cellular events, including developmental gene expression, X chromosome inactivation, genome defense, and genomic imprinting. DNA methylation exerts regulatory functions by recruiting specific binding proteins that contain a highly conserved methyl-CpG binding domain (MBD) [PMID:12610534,16403636]. Methyl-CpG binding domain protein 5 (MBD5) belongs to the MBD family proteins, which play central roles in transcriptional regulation and development. It is a candidate gene involved in human 2q23.1 microdeletion syndrome [PMID:23077600].

Protocols

Product Specific Protocols
WB protocol for MBD5 antibody 15961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
  • KD Validated
humanWB

Genet Test Mol Biomarkers

The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.

Authors - Mei-Rong Liang
mouseIHC

Cell Rep

Distinct Pathogenic Genes Causing Intellectual Disability and Autism Exhibit a Common Neuronal Network Hyperactivity Phenotype.

Authors - Monica Frega
humanIF

Int J Mol Sci

A Novel Genetic Variant in MBD5 Associated with Severe Epilepsy and Intellectual Disability: Potential Implications on Neural Primary Cilia

Authors - Mariana Martins