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  • Featured Product
  • KD/KO Validated

MBIP Polyclonal antibody, PBS Only

MBIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10685-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MBIP Polyclonal antibody (10685-1-AP)

    WB analysis of HepG2 using 10685-1-AP (same clone as 10685-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MBIP Polyclonal antibody (10685-1-AP)

    WB analysis of HEK-293 using 10685-1-AP (same clone as 10685-1-PBS)

    WB result of MBIP antibody (10685-1-AP; 1:600; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells. This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MBIP Polyclonal antibody (10685-1-AP)

    WB analysis of HEK-293 using 10685-1-AP (same clone as 10685-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MBIP Polyclonal antibody (10685-1-AP)

    IP experiment of HEK-293 using 10685-1-AP (same clone as 10685-1-PBS)

    IP result of anti-MBIP (IP:10685-1-AP, 4ug; Detection:10685-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MBIP Polyclonal antibody (10685-1-AP)

    IHC staining of human testis using 10685-1-AP (same clone as 10685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MBIP Polyclonal antibody (10685-1-AP)

    IHC staining of human testis using 10685-1-AP (same clone as 10685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MBIP Polyclonal antibody (10685-1-AP)

    IHC staining of human lung cancer using 10685-1-AP (same clone as 10685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MBIP Polyclonal antibody (10685-1-AP)

    IHC staining of human lung cancer using 10685-1-AP (same clone as 10685-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MBIP Polyclonal antibody (10685-1-AP)

    IF Staining of HepG2 using 10685-1-AP (same clone as 10685-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10685-1-AP (MBIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10685-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10685-1-PBS targets MBIP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBIP fusion protein Ag1061 相同性解析による交差性が予測される生物種
Full Name MAP3K12 binding inhibitory protein 1
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC005197
Gene symbol MBIP
Gene ID (NCBI) 51562
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.