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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MBNL1 Monoclonal antibody (66837-1-Ig)

    WB analysis using 66837-1-Ig (same clone as 66837-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66837-1-Ig (MBNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using MBNL1 Monoclonal antibody (66837-1-Ig)

    WB analysis of rat heart using 66837-1-Ig (same clone as 66837-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66837-1-Ig (MBNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MBNL1 Monoclonal antibody (66837-1-Ig)

    IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66837-1-Ig (MBNL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MBNL1 Monoclonal antibody (66837-1-Ig)

    IHC staining of human breast cancer using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66837-1-Ig (MBNL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MBNL1 Monoclonal antibody (66837-1-Ig)

    IF Staining of HepG2 using 66837-1-Ig (same clone as 66837-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66837-1-Ig (MBNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66837-1-PBS in a different storage buffer formulation.

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MBNL1 Monoclonal antibody, PBS Only

MBNL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66837-1-PBS
Clone No.1H2C12

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp686P06174, EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL, MBNL1, muscleblind like (Drosophila), Muscleblind like protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66837-1-PBS targets MBNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MBNL1 fusion protein Ag7752 相同性解析による交差性が予測される生物種
Full Name muscleblind-like (Drosophila)
Calculated molecular weight 42 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC050535
Gene Symbol MBNL1
Gene ID (NCBI) 4154
RRIDAB_2882180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NR56
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MBNL1 (muscleblind-like 1) is one of the MBNL proteins containing 3 members (MBNL1-3) in vertebrates. And MBNL1 is widely expressed in adult tissues including brain, heart and skeletal muscle (PMID:25274774). As a highly conserved RNA-binding protein, MBNL1 regulates alternative splicing, alternative polyadenylation, mRNA stability and mRNA localization in the nucleus. The neurological diseases DM1 and DM2 (myotonic dystrophy type 1 and type2) are caused by expansion of repetitive sequences in non-coding regions in the DMPK gene because of the inappropriate patterns of alternative splicing owing to MBNL1(PMID:24354850). The antibody could recognize the common full-length protein in applications.