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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MBNL3 Polyclonal antibody

MBNL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24610-1-AP

Synonyms

CHCR, Cys3His CCG1 required protein, MBLX, MBLX39, MBNL3, MBXL, Muscleblind like protein 3, Protein HCHCR

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MBNL3 Polyclonal antibody (24610-1-AP)

    WB analysis of mouse skeletal muscle using 24610-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24610-1-AP (MBNL3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MBNL3 Polyclonal antibody (24610-1-AP)

    WB analysis of mouse skeletal muscle using 24610-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24610-1-AP (MBNL3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MBNL3 Polyclonal antibody (24610-1-AP)

    WB analysis of mouse skeletal muscle using 24610-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24610-1-AP (MBNL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MBNL3 Polyclonal antibody (24610-1-AP)

    IHC staining of human skeletal muscle using 24610-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24610-1-AP (MBNL3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MBNL3 Polyclonal antibody (24610-1-AP)

    IHC staining of human skeletal muscle using 24610-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24610-1-AP (MBNL3 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24610-1-AP is MBNL3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBNL3 fusion protein Ag20113 相同性解析による交差性が予測される生物種
Full Name muscleblind-like 3 (Drosophila)
Calculated molecular weight 354 aa, 39 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC042090
Gene symbol MBNL3
Gene ID (NCBI) 55796
RRIDAB_2879637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MBNL3, also named as Muscleblind-like protein 3 or Cys3His CCG1-required protein, is a 354 amino acid protein, which contains four C3H1-type zinc fingers and belongs to the muscleblind family. MBNL3 localizes in the nucleus and is highly expressed in the placenta. MBNL3 mediates pre-mRNA alternative splicing regulation and plays a role in myotonic dystrophy pathophysiology. MBNL3 could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.

Protocols

Product Specific Protocols
WB protocol for MBNL3 antibody 24610-1-APDownload protocol
IHC protocol for MBNL3 antibody 24610-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.

Authors - Daniel J Foster
View Article