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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MBOAT7 Polyclonal antibody (26646-1-AP)

    WB analysis using 26646-1-AP

    mouse liver tissue was subjected to SDS PAGE followed by western blot with 26646-1-AP (MBOAT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MBOAT7 Polyclonal antibody

MBOAT7 Polyclonal Antibody for WB, ELISA
Cat No. 26646-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

hMBOA 7, h mboa 7, EC:2.3.1.-, Bladder and breast carcinoma-overexpressed gene 1 protein, BB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26646-1-AP targets MBOAT7 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBOAT7 fusion protein Ag24490 相同性解析による交差性が予測される生物種
Full Name membrane bound O-acyltransferase domain containing 7
Calculated molecular weight 472 aa, 53 kDa
Observed molecular weight45 kDa
GenBank accession numberBC006309
Gene Symbol MBOAT7
Gene ID (NCBI) 79143
RRIDAB_3085890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96N66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.

Protocols

Product Specific Protocols
WB protocol for MBOAT7 antibody 26646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols