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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MBOAT7 Recombinant antibody, PBS Only

MBOAT7 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240505E8

Cat no : 83546-3-PBS

Synonyms

h mboa 7, EC:2.3.1.-, Bladder and breast carcinoma-overexpressed gene 1 protein, BB1, 240505E8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using MBOAT7 Recombinant antibody (83546-3-RR)

    IF Staining of HuH-7 using 83546-3-RR (same clone as 83546-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using MBOAT7 Recombinant antibody (83546-3-RR)

    FC experiment of A549 using 83546-3-RR (same clone as 83546-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83546-3-RR

    Affinity and Kinetic Characterization of 83546-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83546-3-PBS is MBOAT7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MBOAT7 fusion protein Ag24466 相同性解析による交差性が予測される生物種
Full Name membrane bound O-acyltransferase domain containing 7
Calculated molecular weight 472 aa, 53 kDa
GenBank accession numberBC006309
Gene symbol MBOAT7
Gene ID (NCBI) 79143
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.