Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A375 cells, mouse lung tissue, HUVEC cells, HepG2 cells |
| Positive IP detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
| Positive IF/ICC detected in | HUVEC cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 8 publications below |
| IHC | See 7 publications below |
| IF | See 11 publications below |
| IP | See 1 publications below |
| FC | See 3 publications below |
Product Information
17564-1-AP targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD146/MCAM fusion protein Ag11762 相同性解析による交差性が予測される生物種 |
| Full Name | melanoma cell adhesion molecule |
| Calculated molecular weight | 646 aa, 72 kDa |
| Observed molecular weight | 113-120 kDa |
| GenBank accession number | BC056418 |
| Gene Symbol | CD146 |
| Gene ID (NCBI) | 4162 |
| RRID | AB_2143373 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43121 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD146/MCAM antibody 17564-1-AP | Download protocol |
| IHC protocol for CD146/MCAM antibody 17564-1-AP | Download protocol |
| IF protocol for CD146/MCAM antibody 17564-1-AP | Download protocol |
| IP protocol for CD146/MCAM antibody 17564-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomaterials Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate. | ||
Oncogene The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.
| ||
Cell Prolif Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo. | ||
Stem Cell Res Ther Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis | ||
J Proteome Res Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.
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