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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCAT Polyclonal antibody (15536-1-AP)

    WB analysis using 15536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15536-1-AP (MCAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MCAT Polyclonal antibody (15536-1-AP)

    IF Staining of HepG2 using 15536-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MCAT antibody (15536-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MCAT Polyclonal antibody

MCAT Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15536-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MCT, Malonyl-CoA-acyl carrier protein transacylase, mitochondrial, FASN2C, fabD, EC:2.3.1.39

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15536-1-AP targets MCAT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCAT fusion protein Ag7867 相同性解析による交差性が予測される生物種
Full Name malonyl CoA:ACP acyltransferase (mitochondrial)
Calculated molecular weight 43 kDa
Observed molecular weight39 kDa
GenBank accession numberBC042195
Gene Symbol MCAT
Gene ID (NCBI) 27349
RRIDAB_3669211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Malonyl-CoA-acyl carrier protein transacylase (MCAT), also known as MT, is an enzyme involved in mitochondrial fatty acid synthesis (mtFAS) and catalyzes the transfer of the malonyl moiety of malonyl-CoA to the mitochondrial acyl carrier protein (ACP) (PMID: 36881526). It has four coding exons and is transcribed into two isoforms by the alternative splicing of exon 3 (PMID: 33918393).

Protocols

Product Specific Protocols
WB protocol for MCAT antibody 15536-1-APDownload protocol
IF protocol for MCAT antibody 15536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols