Validation Data Gallery
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Tested Applications
| Positive WB detected in | fetal human brain tissue, HeLa cells, human brain tissue, L02 cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
11525-1-AP targets MCC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCC fusion protein Ag2089 相同性解析による交差性が予測される生物種 |
| Full Name | mutated in colorectal cancers |
| Calculated molecular weight | 829 aa, 93 kDa |
| Observed molecular weight | 98 kDa, 113-118 kDa |
| GenBank accession number | BC018919 |
| Gene Symbol | MCC |
| Gene ID (NCBI) | 4163 |
| RRID | AB_2143796 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23508 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation a3nd the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MCC antibody 11525-1-AP | Download protocol |
| IHC protocol for MCC antibody 11525-1-AP | Download protocol |
| IF protocol for MCC antibody 11525-1-AP | Download protocol |
| IP protocol for MCC antibody 11525-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene 'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells. | ||
Proteomics Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity. | ||
Front Med (Lausanne) MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.
| ||
PLoS One. Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1. | ||
bioRxiv Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes | ||
Nucleic Acids Res Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes |