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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCCC1 Polyclonal antibody

MCCC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30081-1-AP

Synonyms

MCCA, MCC B, EC:6.4.1.4, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha, 3-methylcrotonyl-CoA carboxylase biotin-containing subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCCC1 Polyclonal antibody (30081-1-AP)

    WB analysis using 30081-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30081-1-AP (MCCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using MCCC1 Polyclonal antibody (30081-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30081-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MCCC1 Polyclonal antibody (30081-1-AP)

    IHC staining of human ovary cancer using 30081-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MCCC1 Polyclonal antibody (30081-1-AP)

    IHC staining of human stomach cancer using 30081-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using MCCC1 Polyclonal antibody (30081-1-AP)

    IF Staining of U-251 using 30081-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MCCC1 antibody (30081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MCCC1 Polyclonal antibody (30081-1-AP)

    IF Staining of MCF-7 using 30081-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCCC1 antibody (30081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, mouse heart tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30081-1-AP targets MCCC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCCC1 fusion protein Ag32459 相同性解析による交差性が予測される生物種
Full Name methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
Calculated molecular weight 76 kDa
Observed molecular weight70 kDa
GenBank accession numberBC004187
Gene symbol MCCC1
Gene ID (NCBI) 56922
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Protocols

Product Specific Protocols
WB protocol for MCCC1 antibody 30081-1-APDownload protocol
IHC protocol for MCCC1 antibody 30081-1-APDownload protocol
IF protocol for MCCC1 antibody 30081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols