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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCHR1 Polyclonal antibody

MCHR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10163-1-AP

Synonyms

G protein coupled receptor 24, GPR24, MCH 1R, MCH R1, MCH receptor 1, MCH1R, MCHR, MCHR 1, MCHR1, SLC 1, SLC1

Validation Data Gallery

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  • Western Blot (WB) analysis of Human anterior cingulate using MCHR1 Polyclonal antibody (10163-1-AP)

    WB analysis of Human anterior cingulate using 10163-1-AP

    Human anterior cingulate tissue were subjected to SDS PAGE followed by western blot with 10163-1-AP (MCHR1 antibody) at dilution of 1:1000.

  • Immunohistochemistry (IHC) staining of human heart tissue using MCHR1 Polyclonal antibody (10163-1-AP)

    IHC staining of human heart using 10163-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10163-1-AP (MCHR1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MCHR1 Polyclonal antibody (10163-1-AP)

    IHC staining of human heart using 10163-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10163-1-AP (MCHR1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHuman anterior cingulate
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10163-1-AP targets MCHR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCHR1 fusion protein Ag0177 相同性解析による交差性が予測される生物種
Full Name melanin-concentrating hormone receptor 1
Calculated molecular weight 46-50 kDa
Observed molecular weight 46 kDa, 35 kDa
GenBank accession numberBC001736
Gene symbol MCHR1
Gene ID (NCBI) 2847
RRIDAB_2143942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for MCHR1 antibody 10163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress.

Authors - Jun Tian
View Article