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  • Featured Product
  • KD/KO Validated

MCL1 Monoclonal antibody, PBS Only (Capture)

MCL1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3C2

Cat no : 66026-1-PBS

Synonyms

Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1, Bcl-2-like protein 3, Bcl2-L-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCL1 Monoclonal antibody (66026-1-Ig)

    WB analysis using 66026-1-Ig (same clone as 66026-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MCL1 Monoclonal antibody (66026-1-Ig)

    WB analysis of Jurkat using 66026-1-Ig (same clone as 66026-1-PBS)

    WB result of MCL1 antibody (66026-1-Ig, 1:1000) with si-Control and si-MCL1 transfected Jurkat cells. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MCL1 Monoclonal antibody (66026-1-Ig)

    WB analysis of Jurkat using 66026-1-Ig (same clone as 66026-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using MCL1 Monoclonal antibody (66026-1-Ig)

    WB analysis of Raji using 66026-1-Ig (same clone as 66026-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MCL1 Monoclonal antibody (66026-1-Ig)

    WB analysis of A549 using 66026-1-Ig (same clone as 66026-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using MCL1 Monoclonal antibody (66026-1-Ig)

    WB analysis of A431 using 66026-1-Ig (same clone as 66026-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MCL1 Monoclonal antibody (66026-1-Ig)

    WB analysis of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using MCL1 Monoclonal antibody (66026-1-Ig)

    WB analysis of A431 using 66026-1-Ig (same clone as 66026-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MCL1 Monoclonal antibody (66026-1-Ig)

    IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MCL1 Monoclonal antibody (66026-1-Ig)

    IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MCL1 Monoclonal antibody (66026-1-Ig)

    IF Staining of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCL1 antibody (66026-1-Ig, Clone: 1E3C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MCL1 Monoclonal antibody (66026-1-Ig)

    IF Staining of MCF-7 using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66026-1-Ig (MCL1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MCL1 Monoclonal antibody (66026-1-Ig)

    IF Staining of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

    Immunofluorescent analysis of Hela cells, using MCL1 antibody 66026-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50655-1

    Cytometric bead array standard curve of MP50655-1

    Cytometric bead array standard curve of MP50655-1, MCL1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66026-1-PBS. Detection antibody: 66026-2-PBS. Standard:Ag10609. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66026-1-PBS targets MCL1 as part of a matched antibody pair:

MP50655-1: 66026-1-PBS capture and 66026-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MCL1 fusion protein Ag10609 相同性解析による交差性が予測される生物種
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Calculated molecular weight 350 aa, 37 kDa
Observed molecular weight35 kDa
GenBank accession numberBC107735
Gene symbol MCL1
Gene ID (NCBI) 4170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.