MCM2 Monoclonal antibody, PBS Only

WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

4T1 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

A431 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

K-562 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (66204-1-Ig, Clone: 1F4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.