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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCM2 Recombinant antibody

MCM2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241968D3

Cat no : 84511-2-RR

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM2 Recombinant antibody (84511-2-RR)

    WB analysis using 84511-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84511-2-RR (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MCM2 Recombinant antibody (84511-2-RR)

    IF Staining of HeLa using 84511-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (84511-2-RR, Clone: 241968D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MCM2 Recombinant antibody (84511-2-RR)

    IF Staining of MCF-7 using 84511-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (84511-2-RR, Clone: 241968D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using MCM2 Recombinant antibody (84511-2-RR)

    FC experiment of HeLa using 84511-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM2 Recombinant antibody (84511-2-RR, Clone:241968D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of MCF-7 cells using MCM2 Recombinant antibody (84511-2-RR)

    FC experiment of MCF-7 using 84511-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MCM2 Recombinant antibody (84511-2-RR, Clone:241968D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84511-2-RR

    Affinity and Kinetic Characterization of 84511-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84511-2-RR against Human MCM2 were performed. The affinity constant is 0.453 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, Jurkat cells, A431 cells
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84511-2-RR targets MCM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MCM2 fusion protein Ag0798 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 2
Calculated molecular weight 102 kDa
Observed molecular weight 116-125 kDa
GenBank accession numberBC007670
Gene symbol MCM2
Gene ID (NCBI) 4171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

Protocols

Product Specific Protocols
WB protocol for MCM2 antibody 84511-2-RRDownload protocol
IF protocol for MCM2 antibody 84511-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols