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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM2 Recombinant antibody (84511-5-RR)

    WB analysis using 84511-5-RR (same clone as 84511-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84511-5-RR (MCM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of mouse colon using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of mouse colon using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of human colon cancer using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of human colon cancer using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of human placenta using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of human placenta using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of mouse small intestine using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of mouse small intestine using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical squamous cancer tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of human cervical squamous cancer using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical squamous cancer tissue using MCM2 Recombinant antibody (84511-5-RR)

    IHC staining of human cervical squamous cancer using 84511-5-RR (same clone as 84511-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MCM2 Recombinant antibody (84511-5-RR)

    IF Staining of MCF-7 using 84511-5-RR (same clone as 84511-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (84511-5-RR, Clone: 241968F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MCM2 Recombinant antibody (84511-5-RR)

    IF Staining of HeLa using 84511-5-RR (same clone as 84511-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (84511-5-RR, Clone: 241968F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84511-5-RR

    Affinity and Kinetic Characterization of 84511-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84511-5-RR against Human MCM2 were performed. The affinity constant is 2.04 nM.

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MCM2 Recombinant antibody, PBS Only

MCM2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84511-5-PBS
Clone No.241968F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84511-5-PBS targets MCM2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MCM2 fusion protein Ag0798 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 2
Calculated molecular weight 102 kDa
Observed molecular weight 116-125 kDa
GenBank accession numberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49736
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.