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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM3 Recombinant antibody (84792-5-RR)

    WB analysis using 84792-5-RR (same clone as 84792-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84792-5-RR (MCM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84792-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MCM3 Recombinant antibody (84792-5-RR)

    IHC staining of human colon cancer using 84792-5-RR (same clone as 84792-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84792-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MCM3 Recombinant antibody (84792-5-RR)

    IHC staining of human colon cancer using 84792-5-RR (same clone as 84792-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84792-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MCM3 Recombinant antibody (84792-5-RR)

    IHC staining of human colon cancer using 84792-5-RR (same clone as 84792-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84792-5-RR (MCM3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84792-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using MCM3 Recombinant antibody (84792-5-RR)

    FC experiment of HeLa using 84792-5-RR (same clone as 84792-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM3 Recombinant antibody (84792-5-RR, Clone:242103B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84792-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using MCM3 Recombinant antibody (84792-5-RR)

    FC experiment of K-562 using 84792-5-RR (same clone as 84792-5-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug MCM3 Recombinant antibody (84792-5-RR, Clone:242103B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84792-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84792-5-RR

    Affinity and Kinetic Characterization of 84792-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84792-5-RR against Human MCM3 were performed. The affinity constant is 73.2 pM.

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MCM3 Recombinant antibody, PBS Only

MCM3 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84792-5-PBS
Clone No.242103B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

P1-MCM3, p102, P1 MCM3, EC:3.6.4.12, DNA polymerase alpha holoenzyme-associated protein P1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84792-5-PBS targets MCM3 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MCM3 fusion protein Ag7966 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 3
Calculated molecular weight 91 kDa
Observed molecular weight 102-105 kDa
GenBank accession numberBC001626
Gene Symbol MCM3
Gene ID (NCBI) 4172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25205
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).