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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM4 Monoclonal antibody (67103-1-Ig)

    WB analysis using 67103-1-Ig (same clone as 67103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using MCM4 Monoclonal antibody (67103-1-Ig)

    WB analysis of HSC-T6 using 67103-1-Ig (same clone as 67103-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using MCM4 Monoclonal antibody (67103-1-Ig)

    WB analysis of NIH/3T3 using 67103-1-Ig (same clone as 67103-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MCM4 Monoclonal antibody (67103-1-Ig)

    IF Staining of U2OS using 67103-1-Ig (same clone as 67103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MCM4 antibody (67103-1-Ig, Clone: 2H2A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

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MCM4 Monoclonal antibody, PBS Only

MCM4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67103-1-PBS
Clone No.2H2A1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

P1 CDC21, hCdc21, EC:3.6.4.12, CDC54, CDC21

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67103-1-PBS targets MCM4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MCM4 fusion protein Ag28642 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 4
Calculated molecular weight 863 aa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC031061
Gene Symbol MCM4
Gene ID (NCBI) 4173
RRIDAB_2882408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33991
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.