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KD/KO Validated

MCM6 Polyclonal antibody

Name: MCM6 Polyclonal antibody
Brand: Proteintech
SKU: 13347-2-AP

MCM6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13347-2-AP

Synonyms

P105MCM, Mis5, MCG40308, EC:3.6.4.12

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 13347-2-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13347-2-AP
WB result of MCM6 antibody (13347-2-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM6 transfected HeLa cells.
WB analysis of mouse ovary using 13347-2-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13347-2-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 13347-2-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13347-2-AP
IP result of anti-MCM6 (IP:13347-2-AP, 3ug; Detection:13347-2-AP 1:1000) with HeLa cells lysate 3800ug.
IHC staining of human cervical cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP (MCM6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP (MCM6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human cervical cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 13347-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 13347-2-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM6 antibody (13347-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 13347-2-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells, mouse ovary tissue, mouse thymus tissue, mouse spleen tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human cervical cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 16 publications below
IHC	See 8 publications below
IF	See 2 publications below

Product Information

13347-2-AP targets MCM6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCM6 fusion protein Ag4180 相同性解析による交差性が予測される生物種
Full Name	minichromosome maintenance complex component 6
Calculated molecular weight	821 aa, 93 kDa
Observed molecular weight	105 kDa
GenBank accession number	BC032374
Gene symbol	MCM6
Gene ID (NCBI)	4175
RRID	AB_2142537
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

Protocols

Product Specific Protocols
WB protocol for MCM6 antibody 13347-2-AP	Download protocol
IHC protocol for MCM6 antibody 13347-2-AP	Download protocol
IF protocol for MCM6 antibody 13347-2-AP	Download protocol
IP protocol for MCM6 antibody 13347-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	J Hematol Oncol Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma Authors - Yexin Tong View Article
human	WB	Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. Authors - Hongyuan Song View Article
human	WB	Elife MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells. Authors - Yuichiro Saito View Article
human	WB	Front Oncol METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway. Authors - Dong-Dong Yang View Article
human	WB	Oncotarget Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. Authors - Yinan Ma View Article
human	WB	Ecotoxicol Environ Saf The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis. Authors - Zemin Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCM6 Polyclonal antibody

MCM6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 13347-2-AP

WB analysis of HeLa using 13347-2-AP

WB analysis of mouse ovary using 13347-2-AP

WB analysis of mouse thymus using 13347-2-AP

WB analysis of mouse spleen using 13347-2-AP

IP experiment of HeLa using 13347-2-AP

IHC staining of human cervical cancer using 13347-2-AP

IHC staining of human cervical cancer using 13347-2-AP

IHC staining of human cervical cancer using 13347-2-AP

IHC staining of human cervical cancer using 13347-2-AP

IHC staining of human lung cancer using 13347-2-AP

IHC staining of human lung cancer using 13347-2-AP

IHC staining of human cervical cancer using 13347-2-AP

IHC staining of human cervical cancer using 13347-2-AP

IHC staining of human cervical cancer using 13347-2-AP

IHC staining of human cervical cancer using 13347-2-AP

IF Staining of HeLa using 13347-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.