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  • Featured Product
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MCM6 Monoclonal antibody

MCM6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E12H1

Cat no : 67989-1-Ig

Synonyms

MCG40308, MCM6, Mis5, P105MCM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM6 Monoclonal antibody (67989-1-Ig)

    WB analysis using 67989-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67989-1-Ig (MCM6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of HeLa cells using MCM6 Monoclonal antibody (67989-1-Ig)

    WB analysis of HeLa using 67989-1-Ig

    WB result of MCM6 antibody (67989-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM6 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MCM6 Monoclonal antibody (67989-1-Ig)

    IHC staining of human cervical cancer using 67989-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67989-1-Ig (MCM6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MCM6 Monoclonal antibody (67989-1-Ig)

    IHC staining of human cervical cancer using 67989-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67989-1-Ig (MCM6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MCM6 Monoclonal antibody (67989-1-Ig)

    IF Staining of HepG2 using 67989-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MCM6 antibody (67989-1-Ig, Clone: 2E12H1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MCM6 Monoclonal antibody (67989-1-Ig)

    IF Staining of HepG2 using 67989-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MCM6 antibody (67989-1-Ig, Clone: 2E12H1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, COLO 320 cells, HepG2 cells, MCF-7 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67989-1-Ig targets MCM6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM6 fusion protein Ag4354 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 6
Calculated molecular weight 821 aa, 93 kDa
Observed molecular weight105 kDa
GenBank accession numberBC032374
Gene symbol MCM6
Gene ID (NCBI) 4175
RRIDAB_2918738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

Protocols

Product Specific Protocols
WB protocol for MCM6 antibody 67989-1-IgDownload protocol
IHC protocol for MCM6 antibody 67989-1-IgDownload protocol
IF protocol for MCM6 antibody 67989-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols