MCM7 Polyclonal antibody

Name: MCM7 Polyclonal antibody
Brand: Proteintech
SKU: 11225-1-AP

MCM7 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11225-1-AP

Synonyms

P1.1 MCM3, MCM2, EC:3.6.4.12, CDC47 homolog, CDC47

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of NIH/3T3 using 11225-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 11225-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11225-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of NIH/3T3 using 11225-1-AP
IP result of anti-MCM7 (IP:11225-1-AP, 4ug; Detection:11225-1-AP 1:2000) with NIH/3T3 cells lysate 1600ug.
IHC staining of human lung cancer using 11225-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human lymphoma using 11225-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:50.
IHC staining of human lymphoma using 11225-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 11225-1-AP (MCM7 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 11225-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 11225-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung cancer using 11225-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung cancer using 11225-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:200 (under 10x lens).
IF Staining of A431 using 11225-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using MCM7 antibody (11225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 11225-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MCM7 (11225-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

-/ -

Cat No. 11225-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, NIH/3T3 cells, Jurkat cells, K-562 cells
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	human lung cancer tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 16 publications below
IHC	See 3 publications below
CoIP	See 1 publications below

Product Information

11225-1-AP targets MCM7 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCM7 fusion protein Ag1730 相同性解析による交差性が予測される生物種
Full Name	minichromosome maintenance complex component 7
Calculated molecular weight	81 kDa
Observed molecular weight	81 kDa
GenBank accession number	BC013375
Gene symbol	MCM7
Gene ID (NCBI)	4176
RRID	AB_2297584
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

Protocols

Product Specific Protocols
WB protocol for MCM7 antibody 11225-1-AP	Download protocol
IHC protocol for MCM7 antibody 11225-1-AP	Download protocol
IF protocol for MCM7 antibody 11225-1-AP	Download protocol
IP protocol for MCM7 antibody 11225-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer Authors - Huaiyuan Liang View Article
human	WB	Nucleic Acids Res DOCK7 protects against replication stress by promoting RPA stability on chromatin. Authors - Ming Gao View Article
human	WB	Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. Authors - Hongyuan Song View Article
human	WB	Elife The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP Authors - Maria Körner View Article
human	WB	Elife MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells. Authors - Yuichiro Saito View Article
human	WB	J Nanobiotechnology Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling. Authors - Na Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCM7 Polyclonal antibody

MCM7 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of NIH/3T3 using 11225-1-AP

WB analysis using 11225-1-AP

WB analysis of MCF-7 using 11225-1-AP

IP experiment of NIH/3T3 using 11225-1-AP

IHC staining of human lung cancer using 11225-1-AP

IHC staining of human lymphoma using 11225-1-AP

IHC staining of human lymphoma using 11225-1-AP

IHC staining of human lung cancer using 11225-1-AP

IHC staining of human lung cancer using 11225-1-AP

IHC staining of human lung cancer using 11225-1-AP

IHC staining of human lung cancer using 11225-1-AP

IF Staining of A431 using 11225-1-AP

FC experiment of HepG2 using 11225-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer

DOCK7 protects against replication stress by promoting RPA stability on chromatin.

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.