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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM8 Polyclonal antibody (16451-1-AP)

    WB analysis using 16451-1-AP (same clone as 16451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16451-1-AP (MCM8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16451-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using MCM8 Polyclonal antibody (16451-1-AP)

    IP experiment of NIH/3T3 using 16451-1-AP (same clone as 16451-1-PBS)

    IP result of anti-MCM8 (IP:16451-1-AP, 4ug; Detection:16451-1-AP 1:3000) with NIH/3T3 cells lysate 1505 ug. This data was developed using the same antibody clone with 16451-1-PBS in a different storage buffer formulation.

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MCM8 Polyclonal antibody, PBS Only

MCM8 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 16451-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16451-1-PBS targets MCM8 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCM8 fusion protein Ag8696 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 8
Calculated molecular weight 880 aa, 98 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC008830
Gene Symbol MCM8
Gene ID (NCBI) 84515
RRIDAB_2142833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJA3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. MCM8 exists some isoforms with MV 94 kDa and 70 kDa.